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Title 

Gram-scale production of ginsenoside F1 using a recombinant bacterial β-glucosidase

Authors 

D S AnC H CuiM Z SiddiqiH S YuF X JinSong-Gun KimW T Im

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2017

Citation 

Journal of Microbiology and Biotechnology

Keywords 

BioconversionGlycoside hydrolase family 3Gram-scaleProtopanaxatriolRare ginsenoside

Abstract 

Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare. Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil 3082T. In a systematic step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1. At an optimized NaCl concentration of 200 mM, the protopanaxatriol saponin mixture (25 mg/ml) was incubated with recombinant BgpA (20 mg/ml) for 3 days in a 2.4 L reaction. Following octadecylsilyl silica gel column chromatography, 9.6 g of F1 was obtained from 60 g of substrate mixture at 95% purity, as assessed by chromatography. These results represent the first report of gramscale F1 production via recombinant enzyme-mediated biotransformation

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1703.03006

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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