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Title 

A liver-specific gene expression panel predicts the differentiation status of In vitro hepatocyte models

Authors 

Dae Soo KimJea Woon RyuMi Young SonJ H OhKyung-Sook ChungSugi LeeJeong Ju LeeJun Ho AhnJu-Sik MinJiwon AhnHyun Mi KangJanghwan KimCho Rok JungNam-Soon KimHyun Soo Cho

Publisher 

Wiley-Blackwell

Issue Date 

2017

Citation 

Hepatology

Abstract 

Alternative cell sources, such as three-dimensional organoids and induced pluripotent stem cell?derived cells, might provide a potentially effective approach for both drug development applications and clinical transplantation. For example, the development of cell sources for liver cell?based therapy has been increasingly needed, and liver transplantation is performed for the treatment for patients with severe end-stage liver disease. Differentiated liver cells and three-dimensional organoids are expected to provide new cell sources for tissue models and revolutionary clinical therapies. However, conventional experimental methods confirming the expression levels of liver-specific lineage markers cannot provide complete information regarding the differentiation status or degree of similarity between liver and differentiated cell sources. Therefore, in this study, to overcome several issues associated with the assessment of differentiated liver cells and organoids, we developed a liver-specific gene expression panel (LiGEP) algorithm that presents the degree of liver similarity as a “percentage.” We demonstrated that the percentage calculated using the LiGEP algorithm was correlated with the developmental stages of in vivo liver tissues in mice, suggesting that LiGEP can correctly predict developmental stages. Moreover, three-dimensional cultured HepaRG cells and human pluripotent stem cell?derived hepatocyte-like cells showed liver similarity scores of 59.14% and 32%, respectively, although general liver-specific markers were detected. Conclusion: Our study describes a quantitative and predictive model for differentiated samples, particularly liver-specific cells or organoids; and this model can be further expanded to various tissue-specific organoids; our LiGEP can provide useful information and insights regarding the differentiation status of in vitro liver models. (Hepatology 2017; 66:1662?1674).

ISSN 

0270-9139

Link 

http://dx.doi.org/10.1002/hep.29324

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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