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Title 

An alcohol dehydrogenase gene from Synechocystis sp. confers salt tolerance in transgenic tobacco

Authors 

So Young YiSeong Sub KuH J SimS K KimJi Hyun ParkJae Il LyuEun Jin SoSo Yeon ChoiJonghyun KimMyung-Suk AhnSuk Weon KimHyunwoo ParkWon Joong JeongY P LimSung Ran MinJang Ryol Liu

Publisher 

Frontiers Media

Issue Date 

2017

Citation 

Frontiers in Plant Science

Keywords 

Alcohol dehydrogenaseCyanobacteriaGreen leaf volatiles (GLVs)PrimingSalt toleranceZ-3-hexenol

Abstract 

Synechocystis salt-responsive gene 1 (sysr1) was engineered for expression in higher plants, and gene construction was stably incorporated into tobacco plants. We investigated the role of Sysr1 [a member of the alcohol dehydrogenase (ADH) superfamily] by examining the salt tolerance of sysr1-overexpressing (sysr1-OX) tobacco plants using quantitative real-time polymerase chain reactions, gas chromatographymass spectrometry, and bioassays. The sysr1-OX plants exhibited considerably increased ADH activity and tolerance to salt stress conditions. Additionally, the expression levels of several stress-responsive genes were upregulated. Moreover, airborne signals from salt-stressed sysr1-OX plants triggered salinity tolerance in neighboring wild-type (WT) plants. Therefore, Sysr1 enhanced the interconversion of aldehydes to alcohols, and this occurrence might affect the quality of green leaf volatiles (GLVs) in sysr1-OX plants. Actually, the Z-3-hexenol level was approximately twofold higher in sysr1-OX plants than in WT plants within 1?2 h of wounding. Furthermore, analyses of WT plants treated with vaporized GLVs indicated that Z-3-hexenol was a stronger inducer of stress-related gene expression and salt tolerance than E-2-hexenal. The results of the study suggested that increased C6 alcohol (Z-3-hexenol) induced the expression of resistance genes, thereby enhancing salt tolerance of transgenic plants. Our results revealed a role for ADH in salinity stress responses, and the results provided a genetic engineering strategy that could improve the salt tolerance of crops.

ISSN 

1664-462X

Link 

http://dx.doi.org/10.3389/fpls.2017.01965

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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