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Title 

Current status and perspectives of genome editing technology for microalgae

Authors 

S JeonJong Min LimHyung Gwan LeeS E ShinN K KangY I ParkHee-Mock OhWon Joong JeongB R JeongY K Chang

Publisher 

BioMed Central

Issue Date 

2017

Citation 

Biotechnology for Biofuels

Keywords 

BiofuelsCRISPR/Cas9Genetic engineeringGenome editingGMO conflictsMicroalgae

Abstract 

Genome editing techniques are critical for manipulating genes not only to investigate their functions in biology but also to improve traits for genetic engineering in biotechnology. Genome editing has been greatly facilitated by engineered nucleases, dubbed molecular scissors, including zinc-finger nuclease (ZFN), TAL effector endonuclease (TALEN) and clustered regularly interspaced palindromic sequences (CRISPR)/Cas9. In particular, CRISPR/Cas9 has revolutionized genome editing fields with its simplicity, efficiency and accuracy compared to previous nucleases. CRISPR/Cas9-induced genome editing is being used in numerous organisms including microalgae. Microalgae have been subjected to extensive genetic and biological engineering due to their great potential as sustainable biofuel and chemical feedstocks. However, progress in microalgal engineering is slow mainly due to a lack of a proper transformation toolbox, and the same problem also applies to genome editing techniques. Given these problems, there are a few reports on successful genome editing in microalgae. It is, thus, time to consider the problems and solutions of genome editing in microalgae as well as further applications of this exciting technology for other scientific and engineering purposes

ISSN 

1754-6834

Link 

http://dx.doi.org/10.1186/s13068-017-0957-z

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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