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Title 

Immortalization of porcine 11β-hydroxysteroid dehydrogenase type 1-transgenic liver cells using SV40 large T antigen

Authors 

Hee Young KangY K ChoiY I JeongK C ChoiS H HyunW S HwangE B Jeung

Publisher 

MDPI AG

Issue Date 

2017

Citation 

International Journal of Molecular Sciences

Keywords 

HepatocytesHSD11B1ImmortalizationPig

Abstract 

Cortisol is a steroid hormone essential to the maintenance of homeostasis that is released in response to stress and low blood glucose concentration. Cortisol is converted from cortisone by 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1). It has been reported that too much cortisol or overexpression of HSD11B1 induces obesity and the insulin resistance that accompanies metabolic syndrome in rodent adipose tissue. In our previous study, HSD11B1-transgenic (TG) fibroblasts were established, and a porcine model was generated by SCNT using those fibroblasts. Hepatocytes overexpressing HSD11B1 were obtained from livers of this porcine model and cultured in vitro. However, the primary hepatocytes were found to have a short life span or low proliferation rate. To overcome these problems, the SV40 large T antigen was transduced into primary HSD11B1-TG hepatocytes, and those cells were immortalized. Immortalized HSD11B1-TG hepatocytes showed restored morphology, more rapid proliferation rate, and more expression of HSD11B1 than primary hepatocytes. As well, these cells kept the hepatic characteristics such as gluconeogenic response to cortisone and increased expression of hepatic makers. The immortalized HSD11B1-TG hepatocytes may be useful for studying traits and potential therapeutic drugs for treatment of metabolic disorders induced by overexpression of HSD11B1.

ISSN 

1422-0067

Link 

http://dx.doi.org/10.3390/ijms18122625

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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