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Title 

Generation of an infectious clone of a new Korean isolate of Apple chlorotic leaf spot virus driven by dual 35S and T7 promoters in a versatile binary vector

Authors 

I H KimJ Y HanI S ChoH JuJae Sun MoonE Y SeoH G KimJ HammondH S Lim

Publisher 

Han Rim Won Publishing Company

Issue Date 

2017

Citation 

Plant Pathology Journal

Keywords 

AgroinfiltrationApple chlorotic leaf spot virusFull-length infectious cDNA cloneIn vitro transcriptionT7 promoter

Abstract 

The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained.

ISSN 

I000-0113

Link 

http://dx.doi.org/10.5423/PPJ.NT.05.2017.0106

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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