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Title 

Acoustic wave-driven functionalized particles for aptamer-based target biomolecule separation

Authors 

R AhmadG DestgeerM AfzalJ ParkH AhmedJ H JungK ParkTae-Sung YoonH J Sung

Publisher 

American Chemical Society

Issue Date 

2017

Citation 

Analytical Chemistry

Abstract 

We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside a continuous flow microchannel and to separate particle-conjugated target proteins from a complex fluid. The acoustofluidic device is composed of an interdigitated transducer that produces high-frequency surface acoustic waves (SAW) and a polydimethylsiloxane (PDMS) microfluidic channel. The SAW interacted with the sample fluid inside the microchannel and deflected particles from their original streamlines to achieve separation. Streptavidin-functionalized polystyrene (PS) microparticles were used to capture aptamer (single-stranded DNA) labeled at one end with a biotin molecule. The free end of the customized aptamer15 (apt15), which was attached to the microparticles via streptavidin-biotin linkage to form the PS-apt15 conjugate, was used to capture the model target protein, thrombin (th), by binding at exosite I to form the PS-apt15-th complex. We demonstrated that the PS-apt15 conjugate selectively captured thrombin molecules in a complex fluid. After the PS-apt15-th complex was formed, the sample fluid was pumped through a PDMS microchannel along with two buffer sheath flows that hydrodynamically focused the sample flow prior to SAW exposure for PS-apt15-th separation from the non-target proteins. We successfully separated thrombin from mCardinal2 and human serum using the proposed acoustofluidic device

ISSN 

0003-2700

Link 

http://dx.doi.org/10.1021/acs.analchem.7b03474

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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