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Title 

A novel core 1 O-linked glycan-specific binding lectin from the fruiting body of Hericium erinaceus

 

노루궁뎅이 버섯에서 신규 코어1 O-글라이칸 결합 렉틴

Authors 

Seonghun Kim

Publisher 

Elsevier

Issue Date 

2018

Citation 

International Journal of Biological Macromolecules

Keywords 

Core 1 O-linked glycanGalβ(1,3)GalNAcHericium erinaceusLectinMushroom

Abstract 

Mucin-type O-glycans are involved in biological functions on the cell surface as well as the glycoproteins and can also be used as specific carbohydrate biomarkers of many diseases. In this study, I purified a novel core 1 O-linked glycan specific lectin, Hericium erinaceus lecin (HeL), from the fruiting body of the mushroom Hericium erinaceus, which is known as the natural source for a sialic acid-binding lectin. Upon optimization of the purification conditions, a sequence of ion exchange, affinity, ion exchange, and size-exclusion chromatography resulted in the highest yield and best quality of lectin without protease activity. The resulting purified HeL is an apparent hexameric protein with a subunit molecular weight of 15 kDa, and a pI of 4.3. In hemagglutination inhibition assay, the purified lectin was only inhibited by glycoproteins containing mucin-type O-glycans and reacted weakly with Galβ(1,3)GalNAc. Glycan array analyses showed that HeL specifically interacts with core 1 O-linked glycans as well as extended O-glycan structures containing sialylation or fucosylation. The glycan binding specificity of HeL is comparable to that of peanut agglutinin for detection of a broader range of extended core 1 O-glycan structures. Taken together, these results provide an efficient and optimized procedure for the purification of HeL from the fruiting body of the mushroom Hericium erinaceus. Moreover, HeL represents a powerful tool for analyzing core 1 and extended core 1 O- glycan structures in diagnosis assays

ISSN 

0141-8130

Link 

http://dx.doi.org/10.1016/j.ijbiomac.2017.http://dx.doi.org/10.018

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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