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Title 

Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

Authors 

Sun Young KangBo Min KimKyung Taek HeoJae-Hyuk JangWon Gon KimYoung-Soo Hong

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2017

Citation 

Journal of Microbiology and Biotechnology

Keywords 

Artificial biosynthesiscaffeoyl-HSLferuloyl-HSLphenylacetyl homoserine lactone

Abstract 

A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1705.05033

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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