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Title 

L-Glycine alleviates furfural-induced growth inhibition during isobutanol production in Escherichia coli

Authors 

H S SongJ M JeonY K ChoiJ Y KimW KimJ J YoonK ParkJungoh AhnHong-Weon LeeY H Yang

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2017

Citation 

Journal of Microbiology and Biotechnology

Keywords 

Lignocellulosebiomassfurfuralglycineisobutanol

Abstract 

Lignocellulose is now a promising raw material for biofuel production. However, the lignin complex and crystalline cellulose require pretreatment steps for breakdown of the crystalline structure of cellulose for the generation of fermentable sugars. Moreover, several fermentation inhibitors are generated with sugar compounds, majorly furfural. The mitigation of these inhibitors is required for the further fermentation steps to proceed. Amino acids were investigated on furfural-induced growth inhibition in E. coli producing isobutanol. Glycine and serine were the most effective compounds against furfural. In minimal media, glycine conferred tolerance against furfural. From the IC50 value for inhibitors in the production media, only glycine could alleviate growth arrest for furfural, where 6 mM glycine addition led to a slight increase in growth rate and isobutanol production from 2.6 to 2.8 g/l under furfural stress. Overexpression of glycine pathway genes did not lead to alleviation. However, addition of glycine to engineered strains blocked the growth arrest and increased the isobutanol production about 2.3-fold

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1705.05020

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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