상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

In vitro and in vivo imaging and tracking of intestinal organoids from human induced pluripotent stem cells

Authors 

Kwang Bo JungHana LeeYe Seul SonJi Hye LeeHyun Soo ChoMi Ok LeeJ H OhJaemin LeeSeokho KimCho Rok JungJanghwan KimMi Young Son

Publisher 

Federation of American Society of Experimental Biology (FASEB)

Issue Date 

2018

Citation 

FASEB Journal

Keywords 

ISXKLF5fluorescence imaginghuman intestinereporter system

Abstract 

Human intestinal organoids (hIOs) derived from human pluripotent stem cells (hPSCs) have immense potential as a source of intestines. Therefore, an efficient system is needed for visualizing the stage of intestinal differentiation and further identifying hIOs derived from hPSCs. Here, 2 fluorescent biosensors were developed based on human induced pluripotent stem cell (hiPSC) lines that stably expressed fluorescent reporters driven by intestine-specific gene promoters Kr?ppel-like factor 5 monomeric Cherry (KLF5mCherry) and intestine-specific homeobox enhanced green fluorescence protein (ISXeGFP). Then hIOs were efficiently induced from those transgenic hiPSC lines in which mCherry? or eGFP?expressing cells, which appeared during differentiation, could be identified in intact living cells in real time. Reporter gene expression had no adverse effects on differentiation into hIOs and proliferation. Using our reporter system to screen for hIO differentiation factors, we identified DMH1 as an efficient substitute for Noggin. Transplanted hIOs under the kidney capsule were tracked with fluorescence imaging (FLI) and confirmed histologically. After orthotopic transplantation, the localization of the hIOs in the small intestine could be accurately visualized using FLI. Our study establishes a selective system for monitoring the in vitro differentiation and for tracking the in vivo localization of hIOs and contributes to further improvement of cell-based therapies and preclinical screenings in the intestinal field.Jung, K. B., Lee,H., Son, Y. S., Lee, J.H.,Cho, H.- S., Lee,M.-O., Oh, J.-H., Lee, J.,Kim,S., Jung,C.-R.,Kim, J.,Son,M.-Y. In vitro and in vivo imaging and tracking of intestinal organoids from human induced pluripotent stem cells

ISSN 

0892-6638

Link 

http://dx.doi.org/10.1096/fj.201700504R

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)