상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Sam5, a coumarate 3-hydroxylase from Saccharothrix espanaensis : new insight into the piceatannol production as a resveratrol 3'-hydroxylase

 

Resveratrol 3'-hydroxylase 효소를 이용한 피세타놀 생산

Authors 

Kyung Taek HeoSun Young KangJae-Hyuk JangYoung-Soo Hong

Publisher 

Wiley

Issue Date 

2017

Citation 

ChemistrySelect

Keywords 

biosynthesiscoumarate 3-hydroxylasepiceatannolresveratrol 3’-hydroxylase

Abstract 

Piceatannol is an important natural stilbene, which show a broad range of biological activities of medicinal interest. In recent studies, the Sam5 enzyme from Saccharothrix espanaensis was found to catalyze the conversion of p-coumaric acid to caffeic acid as a coumarate 3-hydroxylase (C3H). In this report, we show that purified recombinant Sam5 enzyme exhibited 5.7-fold higher resveratrol ortho-hydroxylation (resveratrol 3’-hydroxylase; R3’H) activity to produce piceatannol compared to the C3H activity. The substrate saturation curve of the Sam5 enzyme for resveratrol is far superior to that for p-coumaric acid. In addition, we constructed a biological platform to produce piceatannol by adding a sam5 gene in a resveratrol artificial biosynthetic pathway that was already made. According to the results, piceatannol production as high as 31.5 ± 6.3 mg/L was achieved

ISSN 

2365-6549

Link 

http://dx.doi.org/10.1002/slct.201701969

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)