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Title 

An antibody-immobilized silica inverse opal nanostructure for label-free optical biosensors

Authors 

Wang Sik LeeTaejoon KangS H KimJinyoung Jeong

Publisher 

MDPI AG

Issue Date 

2018

Citation 

Sensors

Keywords 

influenza virusinverse opaloptical biosensorsilica

Abstract 

Three-dimensional SiO2-based inverse opal (SiO2-IO) nanostructures were prepared for use as biosensors. SiO2-IO was fabricated by vertical deposition and calcination processes. Antibodies were immobilized on the surface of SiO2-IO using 3-aminopropyl trimethoxysilane (APTMS), a succinimidyl-[(N-maleimidopropionamido)-tetraethyleneglycol] ester (NHS-PEG4-maleimide) cross-linker, and protein G. The highly accessible surface and porous structure of SiO2-IO were beneficial for capturing influenza viruses on the antibody-immobilized surfaces. Moreover, as the binding leads to the redshift of the reflectance peak, the influenza virus could be detected by simply monitoring the change in the reflectance spectrum without labeling. SiO2-IO showed high sensitivity in the range of 103-105 plaque forming unit (PFU) and high specificity to the influenza A (H1N1) virus. Due to its structural and optical properties, SiO2-IO is a promising material for the detection of the influenza virus. Our study provides a generalized sensing platform for biohazards as various sensing strategies can be employed through the surface functionalization of three-dimensional nanostructures

ISSN 

1424-8220

Link 

http://dx.doi.org/10.3390/s18010307

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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