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Title 

Synthetic biology for microbial heavy metal biosensors

Authors 

H J KimHaeyoung JeongS J Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2018

Citation 

Analytical and Bioanalytical Chemistry

Keywords 

Heavy metalsMicrobial whole-cell biosensorSynthetic biology

Abstract 

Using recombinant DNA technology, various whole-cell biosensors have been developed for detection of environmental pollutants, including heavy metal ions. Whole-cell biosensors have several advantages: easy and inexpensive cultivation, multiple assays, and no requirement of any special techniques for analysis. In the era of synthetic biology, cutting-edge DNA sequencing and gene synthesis technologies have accelerated the development of cell-based biosensors. Here, we summarize current technological advances in whole-cell heavy metal biosensors, including the synthetic biological components (bioparts), sensing and reporter modules, genetic circuits, and chassis cells. We discuss several opportunities for improvement of synthetic cell-based biosensors. First, new functional modules must be discovered in genome databases, and this knowledge must be used to upgrade specific bioparts through molecular engineering. Second, modules must be assembled into functional biosystems in chassis cells. Third, heterogeneity of individual cells in the microbial population must be eliminated. In the perspectives, the development of whole-cell biosensors is also discussed in the aspects of cultivation methods and synthetic cells.

ISSN 

1618-2642

Link 

http://dx.doi.org/10.1007/s00216-017-0751-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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