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Title 

Production of (3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer from coffee waste oil using engineered Ralstonia eutropha

Authors 

S K BhatiaJ H KimM S KimJ KimJ W HongY G HongH J KimJ M JeonS H KimJungoh AhnHong-Weon LeeY H Yang

Publisher 

Springer Verlag (Germany)

Issue Date 

2018

Citation 

Bioprocess and Biosystems Engineering

Keywords 

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)PolyhydroxyalkonateRalstonia eutropha

Abstract 

Polyhydroxyalkonate (PHA) is a type of polymer that has the potential to replace petro-based plastics. To make PHA production more economically feasible, there is a need to find a new carbon source and engineer microbes to produce a commercially valuable polymer. Coffee waste is an inexpensive raw material that contains fatty acids. It can act as a sustainable carbon source and seems quite promising with PHA production in Ralstonia eutropha, which is a well-known microbe for PHA accumulation, and has the potential to utilize fatty acids. In this study, to make poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)), which has superior properties in terms of biodegradability, biocompatibility, and mechanical strength, engineered strain Ralstonia eutropha Re2133 overexpressing (R)-specific enoyl coenzyme-A hydratase (phaJ) and PHA synthetase (phaC2) with deletion of acetoacetyl Co-A reductases (phaB1, phaB2, and phaB3) was used to produce PHA from coffee waste oil. At a coffee oil concentration of 1.5%, and C/N ratio of 20, the R. eutropha Re2133 fermentation process results in 69% w/w of DCW PHA accumulation and consists of HB (78 mol%) and HHx (22 mol%). This shows the feasibility of using coffee waste oil for P(HB-co-HHx) production, as it is a low-cost fatty acid enriched waste material

ISSN 

1615-7591

Link 

http://dx.doi.org/10.1007/s00449-017-1861-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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