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Title 

Analysis of loxoprofen in tablets, patches, and equine urine as tert-butyldimethylsilyl derivative by gas chromatography-mass spectrometry

Authors 

Youngbae KimC SeoS OhJ KwakS JungE SinH KimM JiH S LeeH J ParkG LeeJ YuMinsoo KimW LeeB J Paik

Publisher 

Pharmaceutical Society of Korea

Issue Date 

2018

Citation 

Archives of Pharmacal Research

Keywords 

Equine urineGas chromatography-mass spectrometryLoxoprofenPatchTablettert-Butyldimethylsilyl derivative

Abstract 

Loxoprofen is a non-steroidal anti-inflammatory drug of the 2-arylpropionic acid type, which has used to treat musculoskeletal disorders in the horse racing industry. However, it has also used illicitly to mask clinical signs of inflammation and pain in racehorses. Thus, its accurate analysis has become an important issue in horse doping laboratories. In this study, an analytical method of loxoprofen was developed as tert-butyldimethylsilyl (TBDMS) derivative by gas chromatography-mass spectrometry (GC?MS). Characteristic fragment ions of [M-15], [M-57], and [M-139] permitted the accurate and selective detection of loxoprofen. Under optimal conditions, this method showed good linearity (r ≥ 0.999) in the range of 10?500 ng/mL, repeatability (% relative standard deviation = 5.6?8.5), and accuracy (% relative error = ? 0.3?0.9) with a detection limit of 1.0 ng. When applied to the analysis of loxoprofen in tablet and patch products, loxoprofen was positively identified as TBDMS derivative by GC?MS. The present method provided rapid and accurate determination of loxoprofen in patch and tablet products. Levels of loxoprofen were highest in equine urine at 0.5 and 1 h after oral administration with single dose (3 mg/kg) to three horses, and then rapidly reduced to below the lower limit of quantification at 24 h. Therefore, the present method will be useful for the pharmacokinetic study and doping tests for loxoprofen and other similar acidic drugs in horses

ISSN 

0253-6269

Link 

http://dx.doi.org/10.1007/s12272-018-1023-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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