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Title 

Development and characterization of monoclonal antibodies against nucleoprotein for diagnosis of influenza a virus

Authors 

N H PhuongChaewon KwakChang Kyu HeoEun Wie ChoJihyun YangHaryoung Poo

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2018

Citation 

Journal of Microbiology and Biotechnology

Keywords 

DiagnosisInfluenza A virusMonoclonal antibodyNucleoproteinSensitivity

Abstract 

Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating antiviral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity of those with commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3, and M34.33, exhibited higher reactivities to recombinant NPs and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/ Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed M34.3 and M34.33 mAbs could be useful for the development of influenza diagnostics.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1801.01002

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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