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Title 

Alkaloids from Tetrastigma hemsleyanum and their anti-inflammatory effects on LPS-induced RAW264.7 cells

Authors 

C Y WangHyun-Jae JangY K HanX D SuSeung Woong LeeMun Chual RhoH S WangS Y YangY H Kim

Publisher 

MDPI

Issue Date 

2018

Citation 

Molecules

Keywords 

AlkaloidAnti-inflammatory activityNO productionTetrastigma hemsleyanum

Abstract 

Alkaloids 1-10 were isolated from the aerial parts of Tetrastigma hemsleyanum (APTH) and obtained from species of the genus Tetrastigma for the first time. The chemical structures of the isolated compounds were identified by NMR, UV, and MS analyses. Their anti-inflammatory activities were investigated by measuring nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Among all the isolates, compounds 6, 7 and 10 showed potent inhibitory activity against LPS-stimulated NO production in RAW264.7 cells (IC50: 31.9, 25.2 and 6.3 μM, respectively). Furthermore, APTH and S-(-)-trolline (10) inhibited induction of inflammatory cytokines or mediators such as interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) via suppression of nuclear factor κB (NF- κB) translocation into the nucleus. In addition, 10 suppressed extracellular signal-regulated protein kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) phosphorylation in a dose-dependent manner. These results conclusively demonstrated that compound 10 displays anti-inflammatory activity via suppression of NF- κB activation and the ERK-MAPK signaling pathway in LPS-stimulated RAW264.7 cells

ISSN 

1420-3049

Link 

http://dx.doi.org/10.3390/molecules23061445

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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