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Title 

Effect of BIS depletion on HSF1-dependent transcriptional activation in A549 non-small cell lung cancer cells

Authors 

H H YunJ Y BaekG SeoYong Sam KimJeong Heon KoJ H Lee

Publisher 

The Korean Physiological Society

Issue Date 

2018

Citation 

Korean Journal of Physiology & Pharmacology

Keywords 

A549BCL-2 interacting cell death suppressorHeat shock factor1Heat shock protein

Abstract 

The expression of BCL-2 interacting cell death suppressor (BIS), an antistress or anti-apoptotic protein, has been shown to be regulated at the transcriptional level by heat shock factor 1 (HSF1) upon various stresses. Recently, HSF1 was also shown to bind to BIS, but the significance of these protein-protein interactions on HSF1 activity has not been fully defined. In the present study, we observed that complete depletion of BIS using a CRISPR/Cas9 system in A549 non-small cell lung cancer did not affect the induction of heat shock protein (HSP) 70 and HSP27 mRNAs under various stress conditions such as heat shock, proteotoxic stress, and oxidative stress. The lack of a functional association of BIS with HSF1 activity was also demonstrated by transient downregulation of BIS by siRNA in A549 and U87 glioblastoma cells. Endogenous BIS mRNA levels were significantly suppressed in BIS knockout (KO) A549 cells compared to BIS wild type (WT) A549 cells at the constitutive and inducible levels. The promoter activities of BIS and HSP70 as well as the degradation rate of BIS mRNA were not influenced by depletion of BIS. In addition, the expression levels of the mutant BIS construct, in which 14 bp were deleted as in BIS-KO A549 cells, were not different from those of the WT BIS construct, indicating that mRNA stability was not the mechanism for autoregulation of BIS. Our results suggested that BIS was not required for HSF1 activity, but was required for its own expression, which involved an HSF1-independent pathway.

ISSN 

1226-4512

Link 

http://dx.doi.org/10.4196/kjpp.2018.22.4.457

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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