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Title 

Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos

Authors 

S G YangH J ParkJ W KimJ M JungM J KimH G HegalI S KimM J KangG WeeH Y YangY H LeeJ H SeoSun-Uk KimD B Koo

Publisher 

Nature Publishing Group

Issue Date 

2018

Citation 

Scientific Reports

Abstract 

Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of Mito-TEMPO on porcine embryo development, to our knowledge, has not been studied yet. In the present study, porcine embryos were classified into two groups (G1 and G2) based on the cytoplasm lipid contents at the zygote stage. The development of blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 ± 5.9%; 1.6-fold, p < 0.05) compared to those from G1 zygotes. In G2 embryos, the proportion of TUNEL-positive cells was also higher than that of G1 embryos. Superoxide in G2 embryos was significantly increased compared to that in G1 embryos. Mitochondrial membrane potential and ATP production were lower in G2 embryos than in G1 embryos. Phosphorylation of Drp1 at Ser 616 increased in G1 embryos during the cleavage stages compared to that in the zygote but was not significantly different in G2 embryos. Then, the effects of Mito-TEMPO were investigated in G2 embryos. Blastocyst formation rate (G2: 19.1 ± 5.1% vs G2 + Mito-TEMPO: 28.8 ± 4.0%; 1.5-fold, p < 0.05) and mitochondrial aggregation were recovered after superoxide reduction by Mito-TEMPO treatment. Thus, we showed that Mito-TEMPO improves blastocyst development by superoxide reduction in porcine embryos in vitro

ISSN 

2045-2322

Link 

http://dx.doi.org/10.1038/s41598-018-28497-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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