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Title 

Interleukin-2 induces the in vitro maturation of human pluripotent stem cell-derived intestinal organoids

Authors 

Kwang Bo JungHana LeeYe Seul SonMi Ok LeeYoung-Dae KimS J OhOhman KwonSunwha ChoHyun Soo ChoDae Soo KimJ H OhM ZilbauerJeong Ki MinCho Rok JungJanghwan KimMi Young Son

Publisher 

Nature Publishing Group

Issue Date 

2018

Citation 

Nature Communications

Abstract 

Human pluripotent stem cell (hPSC)-derived intestinal organoids (hIOs) form 3D structures organized into crypt and villus domains, making them an excellent in vitro model system for studying human intestinal development and disease. However, hPSC-derived hIOs still require in vivo maturation to fully recapitulate adult intestine, with the mechanism of maturation remaining elusive. Here, we show that the co-culture with human T lymphocytes induce the in vitro maturation of hIOs, and identify STAT3-activating interleukin-2 (IL-2) as the major factor inducing maturation. hIOs exposed to IL-2 closely mimic the adult intestinal epithelium and have comparable expression levels of mature intestinal markers, as well as increased intestine-specific functional activities. Even after in vivo engraftment, in vitro-matured hIOs retain their maturation status. The results of our study demonstrate that STAT3 signaling can induce the maturation of hIOs in vitro, thereby circumventing the need for animal models and in vivo maturation.

ISSN 

2041-1723

Link 

http://dx.doi.org/10.1038/s41467-018-05450-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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