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Title 

Monitoring of epidermal growth factor degradation productsby high-performance liquid chromatography

Authors 

H KimC SeoM JiYoungbae KimJ D KimD H JeongW LeeM J Paik

Publisher 

The Korean Chemical Society

Issue Date 

2018

Citation 

Bulletin of the Korean Chemical Society

Abstract 

Epidermal growth factor (EGF) has an anti­aging effect of skin. In this study, a HPLC method was developed to monitor of EGF degradation products from pure EGF as a cosmetic raw material. The optimized mobile phase was 7.5 mM sodium phosphate buffer (pH 6.8) at flow rate of 0.6 mL/min. In the method validation of pure EGF, it showed good linearity (r ≥ 0.999) with a detection limit of 0.5 ng/mL. Intra­ and inter­day repeatability as a percentage of relative standard deviation (%RSD) varied from 0.01­2.1, and accuracy was 1.1 as the percentage of relative error (%RE). Under optimized conditions, four peaks of pure EGF and three degradation products were observed after storage at room temperature for 12 days. Pure EGF represented as 97.78 ± 0.03% from the total area of the four peaks, whereas the three unknown peaks showed as 0.53 ± 0.01%, 0.61 ± 0.01%, and 1.08 ± 0.01%, respectively. The present method will be useful for the purity and stability tests of EGF in cosmetic materials and products.

ISSN 

0253-2964

Link 

http://dx.doi.org/10.1002/bkcs.11488

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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