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Title 

A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naive mouse embryonic stem cells

Authors 

S J ChoK T KimJ S KimO S KwonY H GoN Y KangHaejeong HuhMi Rang KimD W HanSung-Hwan MoonY T ChangH J Cha

Publisher 

Elsevier

Issue Date 

2018

Citation 

Biomaterials

Keywords 

Fluorescence probeInner cell massLive isolationNa?ve ESCsPrimed ESCsSlc13a5

Abstract 

Human and mouse embryonic stem cells (ESCs) differ in terms of their pluripotency status, i.e., na?ve vs. primed. This affects various biological properties and leads to several technical hurdles for future clinical applications, such as difficulties in chimera formation, single-cell passaging, and gene editing. In terms of generating functional human tissues and organs via mammalian interspecies chimerism, a fluorescent chemical probe that specifically labels na?ve ESCs would help to isolate these cells and monitor their conversion. This study demonstrates that the fluorescent chemical probe compound of designation yellow 9 (CDy9) selectively stains na?ve, but not primed, mouse ESCs (mESCs). CDy9 entered cells via Slc13a5, a highly expressed membrane transporter in na?ve mESCs. Fluorescence-based cell sorting based on CDy9 staining successfully separated na?ve mESCs from primed mESCs. Mice generated using CDy9+ cells isolated during the conversion of mouse epiblast stem cells into na?ve mESCs exhibited coat color chimerism. Furthermore, CDy9 specifically stained cells in the inner cell mass of mouse embryos. These findings suggest that CDy9 is a useful tool to isolate functional na?ve mESCs.

ISSN 

0142-9612

Link 

http://dx.doi.org/10.1016/j.biomaterials.2018.07.007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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