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Title 

Genome variations of evolved Escherichia coli ET8 witha rhodopsin-based phototrophic metabolism

Authors 

H A KimH J KimM J LeeJ ParkA R ChoiHaeyoung JeongK H JungP KimS J Lee

Publisher 

Wiley

Issue Date 

2018

Citation 

Biotechnology Journal

Keywords 

Gloeobacter rhodopsinadaptive laboratory evolutionchemotrophgalU-disruptionkdtA-upregulationphototroph

Abstract 

We reported that the phototrophic metabolism via plasmid-originated Gloeobacter rhodopsin(GR)-expression is improved in Escherichia coli ET5 harboring pKJ606-GR by a genomic point mutation (dgcQC1082A ) encoding a transmembrane cell signaling protein (Microb. Cell Fact. 16:111, 2017). Another evolved descendant is isolated from the chemostat, and the genome variation of the strain named ET8 harboring pKJ606-GR is investigated in this study. Whole genome sequencing analysis identifies a single point mutation (C3831976A) located in the non-coding upstream region of kdtA and an IS4 insertional mutation at galUG706 without any mutations in the plasmid. ET8 strain shows enhanced kdtA transcription and no growth in the D-galactose or lactose sole carbon sourced minimal media. Size of ET8 strain are almost identical to that of the ancestor. Phototrophic growth and proton pumping in ET8 expressing GR (ET8?+?GR) are increased 1.5-fold and threefold, respectively, compared with those in the ancestor (W3110?+?GR). To verify the effects of the genomic mutations, either the kdtA-upregulation or the galU-disruption is conducted in the ancestor. Both the kdtA-upregulation and the galU-disruption result in the drastic increases of proton-pumping. The physiological properties arising from the genomic variations of the evolved host with the new phototrophic metabolism are further discussed.

ISSN 

1860-6768

Link 

http://dx.doi.org/10.1002/biot.201700497

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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