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Title 

Development of an alcohol-inducible gene expression system for recombinant protein expression in Chlamydomonas reinhardtii

Authors 

Sujin LeeYong Jae LeeSaehae ChoiSu Bin ParkQ G TranJina HeoHee-Sik Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2018

Citation 

Journal of Applied Phycology

Keywords 

Alcohol-inducible promoterChlamydomonas reinhardtiiGene expressionMicroalgae

Abstract 

Microalgae have been widely considered for the production of valuable products, such as lipid-based biofuel, value-added pigments, and anti-photo aging reagents. More recently, microalgae have been considered an alternative host for recombinant protein production because of their economic benefits and ecofriendly characteristics. Additionally, many microalgal strains identified to date are generally recognized as safe (GRAS); therefore, the use of microalgae-based technology is promising. However, basic studies on the genetic engineering of microalgae are rare, despite their importance. Particularly, inducible promoter systems that can be applied for strain engineering or recombinant protein production are rarely studied; hence, a number of challenging issues remain unsolved. Therefore, in this study, we focused on the development of a convenient and compact-inducible promoter system that can be used in microalgae. Based on previous success with plant systems, we employed the alcohol-inducible AlcR-P alcA system, which originates from the filamentous fungus, Aspergillus nidulans. This system comprises only two components, a regulatory protein, AlcR, and an inducible promoter, P alcA. Therefore, construction and transformation of the gene cassettes can be easily performed. Ethanol-dependent gene expression was observed in the transformants with no significant growth retardation or inducer consumption observed in the cells cultivated under optimized conditions.

ISSN 

0921-8971

Link 

http://dx.doi.org/10.1007/s10811-018-1480-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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