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Title 

DSG2 is a functional cell surface marker for identification and isolation of human pluripotent stem cells

Authors 

Jongjin ParkY SonNa Geum LeeKungmin LeeDong Gwang LeeJinhoi SongJaemin LeeSeokho KimMin Ji JoJu Hong JangJangwook LeeJong Gil ParkYeon-Gu KimJang Seong KimJungwoon LeeYee Sook ChoYoung-Jun ParkBaek Soo HanKwang-Hee BaeS HanB KangS HaamSang Hyun LeeSang Chul LeeJeong Ki Min

Publisher 

Elsevier (Cell Press)

Issue Date 

2018

Citation 

Stem Cell Reports

Keywords 

EMTcell surface markerdesmoglein-2monoclonal antibodypluripotent stem cells

Abstract 

Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given the cellular heterogeneity within cultivation and safety concerns, the development of specific and efficient tools to isolate a pure population and eliminate all residual undifferentiated PSCs from differentiated derivatives is a prerequisite for clinical applications. In this study, we raised a monoclonal antibody and identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC (hPSC)-specific cell surface markers, and its expression was rapidly downregulated upon differentiation. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of β-catenin/Slug-mediated epithelial-to-mesenchymal transition. Our results demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.

ISSN 

2213-6711

Link 

http://dx.doi.org/10.1016/j.stemcr.2018.05.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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