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Title 

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3'-overhang

Authors 

Su Bin MoonJeong Mi LeeJeong Gu KangNan Ee LeeDae In HaDo Yon KimSun Hee KimKwangsun YooD KimJeong Heon KoYong Sam Kim

Publisher 

Nature Publishing Group

Issue Date 

2018

Citation 

Nature Communications

Abstract 

Genome editing has been harnessed through the development of CRISPR system, and the CRISPR from Prevotella and Francisella 1 (Cpf1) system has emerged as a promising alternative to CRISPR-Cas9 for use in various circumstances. Despite the inherent multiple advantages of Cpf1 over Cas9, the adoption of Cpf1 has been unsatisfactory because of target-dependent insufficient indel efficiencies. Here, we report an engineered CRISPR RNA (crRNA) for highly efficient genome editing by Cpf1, which includes a 20-base target-complementary sequence and a uridinylate-rich 3'-overhang. When the crRNA is transcriptionally produced, crRNA with a 20-base target-complementary sequence plus a U4AU4 3'-overhang is the optimal configuration. U-rich crRNA also maximizes the utility of the AsCpf1 mutants and multiplexing genome editing using mRNA as the source of multiple crRNAs. Furthermore, U-rich crRNA enables a highly safe and specific genome editing using Cpf1 in human cells, contributing to the enhancement of a genome-editing toolbox.

ISSN 

2041-1723

Link 

http://dx.doi.org/10.1038/s41467-018-06129-w

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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