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Title 

Optimization of Artificial Curcumin Biosynthesis in E. coli by Randomized 5′-UTR Sequences to Control the Multienzyme Pathway

Authors 

Sun Young KangKyung Taek HeoYoung-Soo Hong

Publisher 

American Chemical Society

Issue Date 

2018

Citation 

ACS Synthetic Biology

Keywords 

artificial biosynthesiscurcuminmultienzyme pathwaymultiplex automatic genome engineering

Abstract 

One of the optimization strategies of an artificial biosynthetic metabolic flux with a multienzyme pathway is when the enzyme concentrations are present at the appropriate ratios rather than at their maximum expression. Thus, many recent research efforts have focused on the development of tools that fine-tune the enzyme expression, and these research efforts have facilitated the search for the optimum balance between pathway expression and cell viability. However, the rational approach has some limitations in finding the most optimized expression ratio in in vivo systems. In our study, we focused on fine-tuning the expression level of a six-enzyme reaction for the artificial biosynthesis of curcumin by screening a library of 5'-untranslational region (UTR) sequence mutants made by a multiplex automatic genome engineering (MAGE) tool. From the screening results, a variant (6M08rv) showed about a 38.2-fold improvement in the production of curcumin compared to the parent strain, in which the calculated expression levels of 4-coumarate:CoA ligase (4CL) and phenyldiketide-CoA synthase (DCS), two of the six enzymes, were much lower than those of the parent strain.

ISSN 

2161-5063

Link 

http://dx.doi.org/10.1021/acssynbio.8b00198

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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