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Title 

Low-pH production of D-lactic acid using newly isolated acid tolerant yeast Pichia kudriavzevii NG7

Authors 

Hyun Joo ParkJung Hoon BaeHyeok Jin KoSun Hee LeeBong Hyun SungJ I HanJung Hoon Sohn

Publisher 

Wiley

Issue Date 

2018

Citation 

Biotechnology and Bioengineering

Keywords 

Pichia kudriavzeviiacid tolerancelactic acid (LA)

Abstract 

Lactic acid is a platform chemical for the sustainable production of various materials. To develop a robust yeast platform for low-pH production of d-lactic acid (LA), an acid-tolerant yeast strain was isolated from grape skins and named Pichia kudriavzevii NG7 by ribosomal RNA sequencing. This strain could grow at pH 2.0 and 50°C. For the commercial application of P. kudriavzevii NG7 as a lactic acid producer, the ethanol fermentation pathway was redirected to lactic acid by replacing the pyruvate decarboxylase 1 gene (PDC1) with the d-lactate dehydrogenase gene (d-LDH) derived from Lactobacillus plantarum. To enhance lactic acid tolerance, this engineered strain was adapted to high lactic acid concentrations, and a new transcriptional regulator, PAR1, responsible for acid tolerance, was identified by whole-genome resequencing. The final engineered strain produced 135?g/L and 154?g/L of d-LA with productivity over 3.66?g/L/hr at pH 3.6 and 4.16?g/L/hr at pH 4.7, respectively.

ISSN 

0006-3592

Link 

http://dx.doi.org/10.1002/bit.26745

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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