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Title 

Metabolic comparison between standard medicinal parts and their adventitious roots of Cynanchum wilfordii (Maxim.) Hemsl. using FT-IR spectroscopy after IBA and elicitor treatment

 

IBA 및 elicitor 처리에 따른 백수오 기내 생산 부정근 및 표준품의 FT-IR 스펙트럼 기반 대사체 비교 분석

Authors 

Myung-Suk AhnEun Jin SoEun Yee JieSo Yean ChoiS U ParkB C MoonY M KangSung Ran MinSuk Weon Kim

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2018

Citation 

Journal of Plant Biotechnology

Keywords 

Adventitious rootCynanchum wilfordii (Maxim.) Hemsl.Fourier transform infrared spectroscopy (FT-IR)Partial least square discriminant analysis (PLS-DA)Principal component analysis (PCA)

Abstract 

To determine whether metabolite fingerprinting for whole cell extracts based on Fourier transform infrared spectroscopy (FT-IR) can be used to discriminate and compare metabolic equivalence, standard medicinal parts of Cynanchum wilfordii (Maxim.) Hemsl. and their adventitious roots were subjected to FT-IR. The principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from FT-IR spectral data showed that whole metabolic pattern from the adventitious root of Cynanchum wilfordii was highly similar to its standard medicinal parts. These results clearly showed that mass proliferation of adventitious roots could be applied for the novel supply of standard medicinal parts of medicinal plants. Furthermore, FT-IR spectroscopy combined with multivariate analysis established in this study could be applied as an alternative tool for discriminating of whole metabolic equivalence from standard medicinal parts. Thus, it is proposed that these metabolic discrimination systems from the adventitious root of Cynanchum wilfordii could be applied for metabolic standardization of in vitro grown Cynanchum wilfordii.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2018.45.3.250

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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