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Title 

Fabrication of oligomeric avidin scaffolds for valency-controlled surface display of functional ligands

Authors 

H R YoonH ChoiY A ChoiJ A KimJuyeon JungH M KimY Jung

Publisher 

Wiley

Issue Date 

2018

Citation 

Angewandte Chemie International Edition

Keywords 

avidinmultivalencyprotein engineeringrhizavidinsurface display

Abstract 

Multivalent surface display of biomolecules is crucial to study and utilize multivalent biological interactions. However, precise valency control of surface-displayed ligands remains extremely difficult. Now a series of new oligomeric avidin proteins were fabricated that allow facile control of surface multivalency of biotinylated ligands. Naturally dimeric rhizavidin (RA) was engineered to form a mixture of oligomeric avidin assemblies, and discrete RA oligomers from the dimer to octamer of RA, were homogeneously prepared. These oligomeric avidins are in polygonal forms with expected numbers of stable biotin binding sites. Upon immobilization on low-density biotin-coated gold surfaces, RA dimer, trimer, and tetramer scaffolds provided accurate mean residual valencies of 2, 3, and 4, respectively, for biotinylated proteins. Valency-controlled display of antibody binding protein G on these RA surfaces showed clear valency-dependent enhancement of antibody capturing stability.

ISSN 

1433-7851

Link 

http://dx.doi.org/10.1002/anie.201805749

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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