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Title 

Optimized expression, purification, and rapid detection of recombinant influenza nucleoproteins expressed in Sf9 insect cells

Authors 

Sung Jin YoonYoung-Jun ParkH J KimJ JangS J LeeS KooMooseung Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2018

Citation 

Journal of Microbiology and Biotechnology

Keywords 

InfluenzaSf9 cellsinfluenza nucleoproteinrapid detection test

Abstract 

Accurate and rapid diagnosis of influenza infection is essential to enable early antiviral treatment and reduce the mortality associated with seasonal and epidemic infections. Immunochromatography is one of the most common methods used for the diagnosis of seasonal human influenza; however, it is less effective in diagnosing pandemic influenza virus. Currently, rapid diagnostic kits for pandemic influenza virus rely on the detection of nucleoprotein (NP) or hemagglutinin (HA). NP detection shows higher specificity and is more sensitive than HA detection. In this study, we time-dependently screened expression conditions, and herein report optimal conditions for the expression of recombinant nucleoprotein (rNP), which was 48 h after infection. In addition, we report the use of the expressed rNP in a rapid influenza diagnostic test (SGT i-flex Influenza A&B Test). We constructed expression vectors that synthesized rNP (antigen) of influenza A and B in insect cells (Sf9 cells), employed the purified rNP to the immunoassay test kit, and clearly distinguished NPs of influenza A and influenza B using this rapid influenza diagnostic kit. This approach may improve the development of rapid test kits for influenza using NP.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1805.05053

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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