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Title 

Construction of a Vitreoscilla hemoglobin promoter-based tunable expression system for Corynebacterium glutamicum

 

비트레오실라 헤모글로빈 프로모터 이용 코리네박테리움 글루타미컴 단백질 발현시스템 구축

Authors 

K A BaritugoH T KimM N RhieS Y JoT U KhangK H KangS K SongBinna LeeJae Jun SongJong Hyun ChoiDae-Hee LeeJ C JooS J Park

Publisher 

MDPI

Issue Date 

2018

Citation 

Catalysts

Keywords 

Corynebacterium glutamicumExpression vectorsPvgbSynthetic biologyTunable expression systemVgbVitreoscilla

Abstract 

Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a limited number of tunable systems are available to engineer it for efficient production of platform chemicals. Therefore, in this study, we developed a novel tunable promoter system based on repeats of the Vitreoscilla hemoglobin promoter (Pvgb). Tunable expression of green fluorescent protein (GFP) was investigated under one, four, and eight repeats of Pvgb (Pvgb, Pvgb4, and Pvgb8). The intensity of fluorescence in recombinant C. glutamicum strains increased as the number of Pvgb increased from single to eight (Pvgb8) repeats. Furthermore, we demonstrated the application of the new Pvgb promoter-based vector system as a platform for metabolic engineering of C. glutamicum by investigating 5-aminovaleric acid (5-AVA) and gamma-aminobutyric acid (GABA) production in several C. glutamicum strains. The profile of 5-AVA and GABA production by the recombinant strains were evaluated to investigate the tunable expression of key enzymes such as DavBA and GadBmut. We observed that 5-AVA and GABA production by the recombinant strains increased as the number of Pvgb used for the expression of key proteins increased. The recombinant C. glutamicum strain expressing DavBA could produce higher amounts of 5-AVA under the control of Pvgb8 (3.69 ± 0.07 g/L) than the one under the control of Pvgb (3.43 ± 0.10 g/L). The average gamma-aminobutyric acid production also increased in all the tested strains as the number of Pvgb used for GadBmut expression increased from single (4.81?5.31 g/L) to eight repeats (4.94?5.58 g/L).

ISSN 

2073-4344

Link 

http://dx.doi.org/10.3390/catal8110561

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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