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Title 

Effects of Toona sinensis leaf extract and its chemical constituents on xanthine oxidase activity and serum uric acid levels in potassium oxonate-induced hyperuricemic rats

Authors 

H J YukY S LeeHyung Won RyuS H KimD S Kim

Publisher 

MDPI

Issue Date 

2018

Citation 

Molecules

Keywords 

Toona sinensishyperuricemiaultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometryuric acidxanthine oxidase

Abstract 

Toona sinensis leaf is used as a seasonal vegetable in Korea. A 70% ethanol extract of these leaves exhibited potent xanthine oxidase (XO) inhibition, with a 50% inhibitory concentration (IC50) of 78.4 ?M. To investigate the compounds responsible for this effect, bioassay-guided purification led to the isolation of five constituents, identified as quercetin-3-O-rutinoside, quercetin-3-O-β-d-glucopyranoside, 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (compound 3), quercetin-3-O-α-l-rhamnopyranoside, and kaempferol-3-O-α-l-rhamnopyranoside. Compound 3 showed the most potent inhibition of XO, with an IC50 of 2.8 ?M. This was similar to that of allopurinol (IC50 = 2.3 ?M), which is used clinically to treat hyperuricemia. Kinetic analyses found that compound 3 was a reversible noncompetitive XO inhibitor. In vivo, the T. sinensis leaf extract (300 mg/kg), or compound 3 (40 mg/kg), significantly decreased serum uric acid levels in rats with potassium oxonate-induced hyperuricemia. Furthermore, ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry analysis identified a high level of compound 3 in the leaf extract. These findings suggest that T. sinensis leaves could be developed to produce nutraceutical preparations.

ISSN 

1420-3049

Link 

http://dx.doi.org/10.3390/molecules23123254

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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