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Title 

GmBRC1 is a candidate gene for branching in soybean (Glycine max (L.) Merrill)

 

콩(Glycine max (L.) Merrill)에서 분지를 위한 후보유전자인 GmBRC1

Authors 

S ShimJ HaM Y KimM S ChoiS T KangSoon-Chun JeongJ K MoonS H Lee

Publisher 

MDPI AG

Issue Date 

2019

Citation 

International Journal of Molecular Sciences

Keywords 

BRANCHED1 (BRC1)TCP transcription factorbranchinggenome-wide association study (GWAS)near-isogenic line (NIL)soybean

Abstract 

Branch number is one of the main factors affecting the yield of soybean (Glycine max (L.)). In this study, we conducted a genome-wide association study combined with linkage analysis for the identification of a candidate gene controlling soybean branching. Five quantitative trait nucleotides (QTNs) were associated with branch numbers in a soybean core collection. Among these QTNs, a linkage disequilibrium (LD) block qtnBR6-1 spanning 20 genes was found to overlap a previously identified major quantitative trait locus qBR6-1. To validate and narrow down qtnBR6-1, we developed a set of near-isogenic lines (NILs) harboring high-branching (HB) and low-branching (LB) alleles of qBR6-1, with 99.96% isogenicity and different branch numbers. A cluster of single nucleotide polymorphisms (SNPs) segregating between NIL-HB and NIL-LB was located within the qtnBR6-1 LD block. Among the five genes showing differential expression between NIL-HB and NIL-LB, BRANCHED1 (BRC1; Glyma.06G210600) was down-regulated in the shoot apex of NIL-HB, and one missense mutation and two SNPs upstream of BRC1 were associated with branch numbers in 59 additional soybean accessions. BRC1 encodes TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS 1 and 2 transcription factor and functions as a regulatory repressor of branching. On the basis of these results, we propose BRC1 as a candidate gene for branching in soybean.

ISSN 

1422-0067

Link 

http://dx.doi.org/10.3390/ijms20010135

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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