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Title 

Effect of Veratrum maackii on testosterone propionate-induced benign prostatic hyperplasia in rats

Authors 

H S ParkC S SeoC U B WijerathneH Y JeongOg Sung MoonYoung Won SeoYoung Suk WonH Y SonJ H LimH J Kwun

Publisher 

Pharmaceutical Society of Japan

Issue Date 

2019

Citation 

Biological & Pharmaceutical Bulletin

Keywords 

Veratrum maackiibenign prostatic hyperplasianuclear factor-kappaB (NF-κB)testosterone propionate

Abstract 

Veratrum maackii (VM), a perennial plant in the Melanthiaceae family, has anti-hypertensive, anti-cholinergic, anti-asthmatic, anti-tussive, anti-fungal, anti-melanogenesis, and anti-tumor activities. Here, we investigated the therapeutic effect of VM on benign prostatic hyperplasia (BPH) in human normal prostate cell line (WPMY-1) and a testosterone propionate-induced BPH animal model. WPMY-1 cells were treated with VM (1-10??g/mL) and testosterone propionate (100?nM). BPH in rats was generated via daily subcutaneous injections of testosterone propionate (3?mg/kg) dissolved in corn oil, for 4 weeks. VM (150?mg/kg) was administered daily for 4 weeks by oral gavage concurrently with the testosterone propionate. All rats were sacrificed and the prostates were dissected, weighed, and subjected to histological, immunohistochemical, and biochemical examinations. Immunoblotting experiments indicated that WPMY-1 cells treated testosterone propionate had increased expression of prostate specific antigen (PSA) and androgen receptor (AR), and treatment with VM or finasteride blocked this effect. In rat model, VM significantly reduced prostate weight, prostatic hyperplasia, prostatic levels of dihydrotestosterone (DHT), and expression of proliferation markers such as proliferating cell nuclear antigen (PCNA) and cyclin D1, but increased the expression of pro-apoptotic Bcl-2-associated X protein (Bax) and the cleavage of caspase-3. VM administration also suppressed the testosterone propionate-induced activation of nuclear factor-kappaB (NF-κB). Our results indicate that VM effectively represses the development of testosterone propionate-induced BPH, suggesting it may be a useful treatment agent for BPH.

ISSN 

0918-6158

Link 

http://dx.doi.org/10.1248/bpb.b18-00313

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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