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Title 

Rapid tool for identification of bacterial strains usingFourier transform infrared spectroscopy on genomic DNA

Authors 

Jiyoung LeeMyung-Suk AhnY L LeeEun Yee JieSong-Gun KimSuk Weon Kim

Publisher 

Wiley

Issue Date 

2019

Citation 

Journal of Applied Microbiology

Keywords 

Fourier transform infrared spectroscopybacterial identificationgenomic DNAhierarchical clustering analysismultivariate analysis

Abstract 

AIMS: We developed a new rapid and reliable method for identifying bacteria using a combination of Fourier transform infrared (FT-IR) spectroscopy of bacterial genomic DNA and multivariate analysis. METHODS AND RESULTS: FT-IR spectra of genomic DNA from four type strains of Pseudomonas spp., three type strains of Escherichia spp. and two type strains of Bacillus spp. were analysed in the 4000-400 cm-1 region. Spectral differences were found in the frequency regions of N-H stretching (amide I), C=O stretching vibrations (amide II) and PO2 - ionized asymmetric and symmetric stretching. Partial least squares discriminant analysis of the FT-IR spectra showed that the microbial strains could be discriminated by hierarchical clustering analysis. CONCLUSIONS: FT-IR spectral analysis of bacterial genomic DNA has potential for the rapid identification of bacteria at the genus and species levels. SIGNIFICANCE AND IMPACT OF THE STUDY: This study reports a new bacterial identification method using multivariate analysis of FT-IR spectra of bacterial genomic DNA.

URI 

https://doi.org/10.1111/jam.14171

ISSN 

1364-5072

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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