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Title 

Efficient interleukin-21 production by optimization of codon and signal peptide in Chinese hamster ovarian cells

Authors 

Hee Jun ChoByung Moo OhJong-Tae KimJeewon LimSang Yoon ParkYo Sep HwangKyoung Eun BaekBo Yeon KimIn Pyo ChoiHee Gu Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2019

Citation 

Journal of Microbiology and Biotechnology

Keywords 

CHO cellsIL-21NK cellscodon optimizationsignal peptide

Abstract 

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

URI 

https://doi.org/10.4014/jmb.1811.11042

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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