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Title 

Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats

Authors 

B J KimY H KimM G OhK J KimS E JungJ H JinSun-Uk KimK S MinB Y Ryu

Publisher 

Wiley

Issue Date 

2019

Citation 

Asian Journal of Andrology

Keywords 

germline modificationlentivirusspermatogonial stem celltestistransgenic animal

Abstract 

Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species

URI 

https://doi.org/10.4103/aja.aja_80_18

ISSN 

1008-682X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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