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Title 

Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage

Authors 

E B LeeH M JeonChang-Ung KimS M ParkG ChoH J KimY KimDoo-Jin KimY S KimH LeeJ O Lee

Publisher 

Elsevier

Issue Date 

2019

Citation 

Nanomedicine: Nanotechnology, Biology and Medicine

Keywords 

ConjugationFerritinFlagellinHemagglutininInfluenzaNanoparticles

Abstract 

Hemagglutinin (HA) displayed on a ferritin nano-cage has been shown to be effective in generating a potent immune response against a broad range of influenza infections. Here, we showed that conjugation of flagellin together with HA to the exterior surface of the ferritin cage greatly enhanced not only the humoral immune response in mice but also antigen-specific T cell responses that include Th1 cytokine secretion. The effect of flagellin remained essentially unchanged when the molar ratio of flagellin to HA was reduced from 1:1 to 1:3. Injection of the ferritin-HA-flagellin cage provided protection against lethal virus challenge in mice. We used a small immunoglobulin fragment VL12.3 as a convenient method for attaching HA and flagellin to the ferritin cage. This attachment method can be used for rapid screening of a variety of protein cages and nano-assemblies to identify the most suitable carrier and adjuvant proteins for the target antigen.

URI 

https://doi.org/10.1016/j.nano.2019.01.012

ISSN 

1549-9634

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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