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Title 

Global metabolite profiling based on GC-MS and LC-MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Authors 

Kyong Hee NamDo Young KimHye Jin KimIn Soon PackH J KimY S ChungS Y KimChang-Gi Kim

Publisher 

Springer

Issue Date 

2019

Citation 

Applied Biological Chemistry

Keywords 

Abscisic acidAbscisic acid-responsive element-binding factor 3DroughtGas chromatography?mass spectrometryLiquid chromatography?tandem mass spectrometryMetaboliteSoybean (Glycine max)

Abstract 

Abscisic acid (ABA) is a phytohormone that plays an important role in the adaptive responses to abiotic stresses. We examined the metabolic changes in transgenic soybean that over-expressed Arabidopsis ABA responsive element-binding factor 3 (ABF3), which participates in drought tolerance. Transgenic and non-transgenic plants were exposed to a water deficit, and their metabolic differences were verified by untargeted GC?MS and LC?MS/MS analyses. A total of 64 and 476 primary and secondary metabolites from leaf extracts were identified based on GC?MS and LC?MS/MS platforms, respectively. Principal component analysis derived from both GC?MS and LC?MS/MS data showed a clearly greater separation in the metabolite profiles among three different degrees of drought stress. However, no discrimination of metabolites between transgenic and non-transgenic plants was apparent. Furthermore, except for some free amino acids, quantitative differences in relative levels of those metabolites were less than 50% between genotypes. These results suggest that, during periods of drought, overexpression of ABF3 in transgenic soybean might result in a negligible variance in primary and secondary metabolism when compared with its non-transgenic counterpart.

URI 

https://doi.org/10.1186/s13765-019-0425-5

ISSN 

2468-0834

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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