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Title 

Microbial synthesis of linoleate 9 S-lipoxygenase derived plant C18 oxylipins from C18 polyunsaturated fatty acids

Authors 

Jung Ung AnI G LeeY J KoD K Oh

Publisher 

American Chemical Society

Issue Date 

2019

Citation 

Journal of Agricultural and Food Chemistry

Keywords 

biotransformationepoxy hydroxy fatty acidtrihydroxy fatty acidmicrobial enzymeenzyme catalysis

Abstract 

Plant oxylipins, including hydroxy fatty acids, epoxy hydroxy fatty acids, and trihydroxy fatty acids, which are biosynthesized from C18 polyunsaturated fatty acids (PUFAs), are involved in pathogen-specific defense mechanisms against fungal infections. However, their quantitative biotransformation by plant enzymes has not been reported. A few bacteria produce C18 trihydroxy fatty acids, but the enzymes and pathways related to the biosynthesis of plant oxylipins in bacteria have not been reported. In this study, we first report the biotransformation of C18 PUFAs into plant C18 oxylipins by expressing linoleate 9S-lipoxygenase with and without epoxide hydrolase from the proteobacterium Myxococcus xanthus in recombinant Escherichia coli. Among the nine types of plant oxylipins, 12,13-epoxy-14-hydroxy-cis,cis-9,15-octadecadienoic acid was identified as a new compound by NMR analysis, and 9,10,11-hydroxy-cis,cis-6,12-octadecadienoic acid and 12,13,14-trihydroxy-cis,cis-9,15- octadecadienoic were suggested as new compounds by LC-MS/MS analysis. This study shows that bioactive plant oxylipins can be produced by microbial enzymes.

URI 

https://doi.org/10.1021/acs.jafc.8b05857

ISSN 

0021-8561

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


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