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Title 

Dereplication of components coupled with HPLC-qTOF-MS in the active rraction of Humulus japonicus and it's protective effects against Parkinson's disease mouse model

Authors 

H J LeeB DhodaryJ Y LeeJ P AnYoung-Kyoung RyuKyoung Shim KimChul Ho LeeW K Oh

Publisher 

MDPI

Issue Date 

2019

Citation 

Molecules

Keywords 

Humulus japonicusHPLC-qTOF-MSin vitro MAO B assayin vivo 6-OHDA-lesioned PD animal testParkinson’s Disease

Abstract 

Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory e ects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model.

URI 

https://doi.org/10.3390/molecules24071435

ISSN 

1420-3049

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


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