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Title 

C1 compound biosensors: design, functional study, and applications

 

C1 화합물 바이오센서: 디자인, 기능 연구 및 응용

Authors 

Jin-Young LeeBong Hyun SungSo-Hyung OhKil Koang KwonHyewon LeeHaseong KimDae-Hee LeeSoo Jin YeomSeung Goo Lee

Publisher 

MDPI AG

Issue Date 

2019

Citation 

International Journal of Molecular Sciences

Keywords 

C1 compound biosensorgenetic enzyme screening systemone-carbon compound

Abstract 

The microbial assimilation of one-carbon (C1) gases is a topic of interest, given that products developed using this pathway have the potential to act as promising substrates for the synthesis of valuable chemicals via enzymatic oxidation or C?C bonding. Despite extensive studies on C1 gas assimilation pathways, their key enzymes have yet to be subjected to high-throughput evolution studies on account of the lack of an e cient analytical tool for C1 metabolites. To address this challenging issue, we attempted to establish a fine-tuned single-cell?level biosensor system constituting a combination of transcription factors (TFs) and several C1-converting enzymes that convert target compounds to the ligand of a TF. This enzymatic conversion broadens the detection range of ligands by the genetic biosensor systems. In this study, we presented new genetic enzyme screening systems (GESSs) to detect formate, formaldehyde, and methanol from specific enzyme activities and pathways, named FA-GESS, Frm-GESS, and MeOH-GESS, respectively. All the biosensors displayed linear responses to their respective C1 molecules, namely, formate (1.0?250 mM), formaldehyde (1.0?50 M), and methanol (5?400 mM), and they did so with high specificity. Consequently, the helper enzymes, including formaldehyde dehydrogenase and methanol dehydrogenase, were successfully combined to constitute new versatile combinations of the C1-biosensors.

URI 

https://doi.org/10.3390/ijms20092253

ISSN 

1422-0067

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


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