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Title 

Detection of ampicillin-resistant E. coli using novel nanoprobe-combined fluorescence in situ hybridization

Authors 

Wang Sik LeeSoohyun LeeTaejoon KangChoong-Min RyuJinyoung Jeong

Publisher 

MDPI

Issue Date 

2019

Citation 

Nanomaterials

Keywords 

antibiotic-resistanceampicillinbacteriafluorescence in situ hybridizationnanoprobe

Abstract 

Antibiotic-resistant bacteria present a global threat because the infections they cause are di cult to treat. Therefore, it is highly important to develop advanced methods for the identification of antibiotic resistance gene in the virulent bacteria. Here, we report the development of novel nanoprobes for fluorescence in situ hybridization (FISH) and the application of the nanoprobe to the detection of ampicillin-resistant Escherichia coli. The nanoprobe for FISH was synthesized by the modified sol?gel chemistry and the synthesized nanoprobe provided strong fluorescent signals and pH stability even under natural light condition. For the double-identification of bacteria species and ampicillin-resistance with a single probe in situ, the nanoprobes were conjugated to the two kinds of biotinylated probe DNAs; one for E. coli-species specific gene and the other for a drug-resistant gene. By using the nanoprobe-DNA conjugants, we successfully detected the ampicillin-resistant E. coli through the FISH technique. This result suggests the new insight into light stable FISH application of the nanoprobe for a pathogenic antibiotic-resistance bacterium.

URI 

https://doi.org/10.3390/nano9050750

ISSN 

2079-4991

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


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