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Title 

Functional fragments of AIMP1-derived peptide (AdP) and optimized hydrosol for their topical deposition by Box-behnken design

Authors 

J J LeeY M HanT W KwonD H KimH S LeeW J JungJ KimS KangS K KimC W ChoKyeong-Ryoon LeeD D KimM C ParkJ Y Lee

Publisher 

MDPI

Issue Date 

2019

Citation 

Molecules

Keywords 

AIMP1-derived peptide (AdP)cosmeceutical peptideBox-Behnken designhydrosoltopical delivery of peptides

Abstract 

Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this study, FA and MI domains of AdP (FA-AdP and MI-AdP, respectively) were determined by functional domain mapping, where the activities of several fragments of AdP on fibroblast and melanocyte were tested, and a hydrosol-based topical delivery system for these AdP fragments was prepared. The excipient composition of the hydrosol was optimized to maximize the viscosity and drying rate by using Box-Behnken design. The artificial skin deposition of FA-AdP-loaded hydrosol was evaluated using Keshary-Chien di usion cells equipped with Strat-M membrane (STM). The quantification of the fluorescent dye-tagged FA-AdP in STM was carried out by near-infrared fluorescence imaging. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP (p < 0.05). This work suggests that FA- and MI-AdP are active-domains for anti-wrinkle and whitening activities, respectively, and the hydrosol could be used as a promising cosmetic formulation for the delivery of AdPs to the skin.

URI 

https://doi.org/10.3390/molecules24101967

ISSN 

1420-3049

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


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