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Title 

Surface-independent and oriented immobilization of antibody via one-step polydopamine/protein G coating: application to influenza virus immunoassay

Authors 

Jeong MoonJihyun ByunHongki KimJinyoung JeongEun-Kyung LimJuyeon JungS ChoW K ChoTaejoon Kang

Publisher 

Wiley

Issue Date 

2019

Citation 

Macromolecular Bioscience

Keywords 

antibody immobilizationimmunoassaypolydopamineprotein Gsurface modification

Abstract 

For the construction of high-performance biosensor, it is important to interface bioreceptors with the sensor surface densely and in the optimal orientation. Herein, a simple surface modification method that can optimally immobilize antibodies onto various kinds of surfaces is reported. For the surface modification, a mixture of polydopamine (PDA) and protein G was employed. PDA is a representative mussel-inspired polymer, and protein G is an immunoglobulin-binding protein that enables an antibody to have an optimal orientation. The surface characteristics of PDA/Protein G mixturecoated substrates are analyzed and the PDA/protein G ratio is optimized to maximize the antibody binding efficiency. Moreover, the antibody-immobilized substrates are applied to the detection of influenza viruses with the naked eye, providing a detection limit of 2.9 × 103 pfu mL-1. Importantly, the several substrates (glass, SiO2, Si, Al2O3, polyethylene terephthalate, polyethylene, polypropylene, and paper) can be modified by simple incubation with the mixture of PDA/protein G, and then the anti-influenza A H1N1 antibodies can be immobilized on the substrates successfully. Regardless of the substrate, the influenza viruses are detectable after the sandwich immunoreaction and silver enhancement procedure. It is anticipated that the developed PDA/protein G coating method will extend the range of applicable materials for biosensing.

URI 

https://doi.org/10.1002/mabi.201800486

ISSN 

1616-5187

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


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