상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Spastin contributes to neural development through the regulation of microtubule dynamics in the primary cilia of neural stem cells

Authors 

Bohyeon JeongTae Hwan KimDae Soo KimW H ShinJae-Ran LeeNam-Soon KimDa Yong Lee

Publisher 

Elsevier

Issue Date 

2019

Citation 

Neuroscience

Keywords 

spastinneural stem cellbrain developmentprimary ciliamicrotubule.

Abstract 

Spastin is a microtubule-severing enzyme encoded by SPAST, which is broadly expressed in various cell types originated from multiple organs. Even though SPAST is well known as a regulator of the axon growth and arborization in neurons and a genetic factor of hereditary spastic paraplegia, it also takes part in a wide range of other cellular functions including the regulation of cell division and proliferation. In this study, we investigated a novel biological role of spastin in developing brain using Spast deficient mouse embryonic neural stem cells (NSCs) and perinatal mouse brain. We found that the expression of spastin begins at early embryonic stages in mouse brain. Using Spast shRNA treated NSCs and mouse brain, we showed that Spast deficiency leads to decrease of NSC proliferation and neuronal lineage differentiation. Finally, we found that spastin controls NSC proliferation by regulating microtubule dynamics in primary cilia. Collectively, these data demonstrate that spastin controls brain development by the regulation of NSC functions at early developmental stages.

URI 

https://doi.org/10.1016/j.neuroscience.2019.05.024

ISSN 

0306-4522

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-07-10


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)