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Title 

Poly-γ-glutamic acid complexed with alum induces cross-protective immunity of pandemic H1N1 vaccine

Authors 

Q T NguyenChaewon KwakWang Sik LeeJaemoo KimJinyoung JeongM H SungJihyun YangHaryoung Poo

Publisher 

Frontiers Media

Issue Date 

2019

Citation 

Frontiers in Immunology

Keywords 

vaccine adjuvant, influenza virus, efficacy, cross-protection, antibody-dependent cellular cytotoxicity, cytotoxic T lymphocyte activity

Abstract 

The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases.

URI 

https://doi.org/10.3389/fimmu.2019.01604

ISSN 

1664-3224

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-10-29


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