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Title 

Comparative evaluation of hormones and hormone-like molecule in lineage specification of human induced pluripotent stem cells

Authors 

Seon A ChoiJu-Hyun AnSeung Hwan LeeGeun-Hui LeeHae Jun YangPil Soo JeongJae Jin ChaSanghoon LeeYoung-Ho ParkBong-Seok SongBo Woong SimYoung-Hyun KimJi-Su KimYeung Bae JinJae Won HuhSang-Rae LeeJong Hee LeeSun-Uk Kim

Publisher 

Korean Society for Stem Cell Research

Issue Date 

2019

Citation 

International Journal of Stem Cells

Keywords 

Cell fate decisionEstradiol-17βHematopoietic differentiationHuman induced pluripotent stem cellsRetinoic acidlineage specification

Abstract 

BACKGROUND AND OBJECTIVES: Proficient differentiation of human pluripotent stem cells (hPSCs) into specific lineages is required for applications in regenerative medicine. A growing amount of evidences had implicated hormones and hormone-like molecules as critical regulators of proliferation and lineage specification during in vivo development. Therefore, a deeper understanding of the hormones and hormone-like molecules involved in cell fate decisions is critical for efficient and controlled differentiation of hPSCs into specific lineages. Thus, we functionally and quantitatively compared the effects of diverse hormones (estradiol 17-β (E2), progesterone (P4), and dexamethasone (DM)) and a hormone-like molecule (retinoic acid (RA)) on the regulation of hematopoietic and neural lineage specification. METHODS AND RESULTS: We used 10 nM E2, 3 ?M P4, 10 nM DM, and 10 nM RA based on their functional in vivo developmental potential. The sex hormone E2 enhanced functional activity of hematopoietic progenitors compared to P4 and DM, whereas RA impaired hematopoietic differentiation. In addition, E2 increased CD34+CD45+ cells with progenitor functions, even in the CD43- population, a well-known hemogenic marker. RA exhibited lineage-biased potential, preferentially committing hPSCs toward the neural lineage while restricting the hematopoietic fate decision. CONCLUSIONS: Our findings reveal unique cell fate potentials of E2 and RA treatment and provide valuable differentiation information that is essential for hPSC applications.

URI 

https://doi.org/10.15283/ijsc18137

ISSN 

2005-3606

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-10-29


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